Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 414236..414807 Replicon chromosome
Accession NZ_CP074109
Organism Enterococcus faecium strain VBR48

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JVU00_RS01960 Protein ID WP_002286801.1
Coordinates 414466..414807 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JVU00_RS01955 Protein ID WP_002323011.1
Coordinates 414236..414466 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JVU00_RS01930 (JVU00_01930) 409718..411046 + 1329 WP_002327663.1 FAD-containing oxidoreductase -
JVU00_RS01935 (JVU00_01935) 411068..411694 + 627 WP_002286816.1 cysteine hydrolase -
JVU00_RS01940 (JVU00_01940) 411877..412458 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
JVU00_RS01945 (JVU00_01945) 412823..413397 + 575 Protein_385 SOS response-associated peptidase -
JVU00_RS01950 (JVU00_01950) 413602..413940 - 339 WP_002306002.1 hypothetical protein -
JVU00_RS01955 (JVU00_01955) 414236..414466 + 231 WP_002323011.1 hypothetical protein Antitoxin
JVU00_RS01960 (JVU00_01960) 414466..414807 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JVU00_RS01965 (JVU00_01965) 415163..416325 + 1163 WP_220055651.1 IS3 family transposase -
JVU00_RS01970 (JVU00_01970) 416626..417144 + 519 WP_002286782.1 phosphatase PAP2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T201466 WP_002286801.1 NZ_CP074109:414466-414807 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T201466 NZ_CP098439:c88954-88538 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT201466 WP_002323011.1 NZ_CP074109:414236-414466 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT201466 NZ_CP098439:c89181-88951 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References