Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 414674..415245 Replicon chromosome
Accession NZ_CP074107
Organism Enterococcus faecium strain VBO39

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JVT96_RS01965 Protein ID WP_002286801.1
Coordinates 414904..415245 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JVT96_RS01960 Protein ID WP_002323011.1
Coordinates 414674..414904 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JVT96_RS01935 (JVT96_01935) 410156..411484 + 1329 WP_002327663.1 FAD-containing oxidoreductase -
JVT96_RS01940 (JVT96_01940) 411506..412132 + 627 WP_002286816.1 cysteine hydrolase -
JVT96_RS01945 (JVT96_01945) 412315..412896 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
JVT96_RS01950 (JVT96_01950) 413261..413835 + 575 Protein_386 SOS response-associated peptidase -
JVT96_RS01955 (JVT96_01955) 414040..414378 - 339 WP_002306002.1 hypothetical protein -
JVT96_RS01960 (JVT96_01960) 414674..414904 + 231 WP_002323011.1 hypothetical protein Antitoxin
JVT96_RS01965 (JVT96_01965) 414904..415245 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JVT96_RS01970 (JVT96_01970) 415601..416763 + 1163 WP_086956687.1 IS3 family transposase -
JVT96_RS01975 (JVT96_01975) 417064..417582 + 519 WP_002286782.1 phosphatase PAP2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T201464 WP_002286801.1 NZ_CP074107:414904-415245 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T201464 NZ_CP098438:c4627329-4627018 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT201464 WP_002323011.1 NZ_CP074107:414674-414904 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT201464 NZ_CP098438:c4627017-4626727 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References