Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 415062..415633 Replicon chromosome
Accession NZ_CP074105
Organism Enterococcus faecium strain VBO96

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JVI88_RS01965 Protein ID WP_002286801.1
Coordinates 415292..415633 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JVI88_RS01960 Protein ID WP_002323011.1
Coordinates 415062..415292 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JVI88_RS01935 (JVI88_01935) 410544..411872 + 1329 WP_002327663.1 FAD-containing oxidoreductase -
JVI88_RS01940 (JVI88_01940) 411894..412520 + 627 WP_002286816.1 cysteine hydrolase -
JVI88_RS01945 (JVI88_01945) 412703..413284 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
JVI88_RS01950 (JVI88_01950) 413649..414223 + 575 Protein_386 SOS response-associated peptidase -
JVI88_RS01955 (JVI88_01955) 414428..414766 - 339 WP_002306002.1 hypothetical protein -
JVI88_RS01960 (JVI88_01960) 415062..415292 + 231 WP_002323011.1 hypothetical protein Antitoxin
JVI88_RS01965 (JVI88_01965) 415292..415633 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JVI88_RS01970 (JVI88_01970) 415989..417151 + 1163 WP_086956687.1 IS3 family transposase -
JVI88_RS01975 (JVI88_01975) 417452..417970 + 519 WP_002286782.1 phosphatase PAP2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T201462 WP_002286801.1 NZ_CP074105:415292-415633 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T201462 NZ_CP098438:c4176606-4176322 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT201462 WP_002323011.1 NZ_CP074105:415062-415292 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT201462 NZ_CP098438:c4176838-4176596 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References