Detailed information of TA system TA201460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1353350..1353986
• Replicon: chromosome
• Accession: NZ_CP074101
• Organism: Bacillus sp. FS02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K2MHT4
• Locus tag: KFQ09_RS07020
• Protein ID: WP_003214169.1
• Coordinates: 1353350..1353700 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W8QJ31
• Locus tag: KFQ09_RS07025
• Protein ID: WP_003214273.1
• Coordinates: 1353705..1353986 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ09_RS06980	1348381..1348980	-	600	WP_255836144.1	PP2C family serine/threonine-protein phosphatase	-
KFQ09_RS06985	1348980..1349768	-	789	WP_012009000.1	RNA polymerase sigma factor SigB	-
KFQ09_RS06990	1349734..1350222	-	489	WP_007496471.1	anti-sigma B factor RsbW	-
KFQ09_RS06995	1350219..1350548	-	330	WP_008346902.1	anti-sigma factor antagonist	-
KFQ09_RS07000	1350608..1351615	-	1008	WP_008346904.1	PP2C family protein-serine/threonine phosphatase	-
KFQ09_RS07005	1351626..1352027	-	402	WP_019744214.1	anti-sigma regulatory factor	-
KFQ09_RS07010	1352030..1352398	-	369	WP_003214235.1	RsbT antagonist protein RsbS	-
KFQ09_RS07015	1352403..1353233	-	831	WP_008346908.1	RsbT co-antagonist protein RsbRA	-
KFQ09_RS07020	1353350..1353700	-	351	WP_003214169.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KFQ09_RS07025	1353705..1353986	-	282	WP_003214273.1	hypothetical protein	Antitoxin
KFQ09_RS07030	1354280..1355461	-	1182	WP_241511749.1	alanine racemase	-
KFQ09_RS07035	1355590..1356606	-	1017	WP_007496489.1	outer membrane lipoprotein-sorting protein	-
KFQ09_RS07040	1356767..1357132	-	366	WP_017358398.1	holo-ACP synthase	-
KFQ09_RS07045	1357227..1357832	+	606	WP_007496491.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.99 Da
• Isoelectric Point: 5.1663

>T201460 WP_003214169.1 NZ_CP074101:c1353700-1353350 [Bacillus sp. FS02]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T201460 NZ_CP098438:3671250-3671525 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10864.25 Da
• Isoelectric Point: 5.2726

>AT201460 WP_003214273.1 NZ_CP074101:c1353986-1353705 [Bacillus sp. FS02]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT201460 NZ_CP098438:c3671515-3671093 [Salmonella enterica subsp. enterica serovar Typhimurium]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: K2MHT4

Antitoxin

• AlphaFold DB: A0A081L854