Detailed information of TA system TA201429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3838199..3838959
• Replicon: chromosome
• Accession: NZ_CP074096
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain 2B_cip

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5H3Y7E3
• Locus tag: KFV00_RS18675
• Protein ID: WP_023225088.1
• Coordinates: 3838199..3838684 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A3Z2K6C6
• Locus tag: KFV00_RS18680
• Protein ID: WP_072106018.1
• Coordinates: 3838672..3838959 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV00_RS18645 (3833269)	3833269..3833931	+	663	WP_000747548.1	OmpA family lipoprotein	-
KFV00_RS18650 (3834150)	3834150..3835124	+	975	WP_023225087.1	glyoxylate/hydroxypyruvate reductase GhrB	-
KFV00_RS18655 (3835174)	3835174..3835884	-	711	WP_000190524.1	DUF3053 domain-containing protein	-
KFV00_RS18660 (3836323)	3836323..3836613	+	291	WP_000455790.1	HTH-type transcriptional regulator	-
KFV00_RS18665 (3836902)	3836902..3837114	+	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
KFV00_RS18670 (3837288)	3837288..3837827	+	540	WP_000047141.1	copper-binding periplasmic metallochaperone CueP	-
KFV00_RS18675 (3838199)	3838199..3838684	-	486	WP_023225088.1	GNAT family N-acetyltransferase	Toxin
KFV00_RS18680 (3838672)	3838672..3838959	-	288	WP_072106018.1	DUF1778 domain-containing protein	Antitoxin
KFV00_RS18685 (3839148)	3839148..3839615	-	468	WP_000702452.1	GNAT family N-acetyltransferase	-
KFV00_RS18690 (3839760)	3839760..3840107	-	348	Protein_3671	IS3 family transposase	-
KFV00_RS18695 (3840260)	3840260..3842329	-	2070	WP_023225090.1	glycine--tRNA ligase subunit beta	-
KFV00_RS18700 (3842339)	3842339..3843250	-	912	WP_001168551.1	glycine--tRNA ligase subunit alpha	-
KFV00_RS18705 (3843389)	3843389..3843691	-	303	WP_023206518.1	YsaB family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17756.43 Da
• Isoelectric Point: 9.0965

>T201429 WP_023225088.1 NZ_CP074096:c3838684-3838199 [Salmonella enterica subsp. enterica serovar Typhimurium]
VGHVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHEKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T201429 NZ_CP098403:c737850-737581 [Lactobacillus kefiranofaciens subsp. kefirgranum]
ATGATTTTAAAATATCATGGCAGGATTAAAAACTCAGCTAAGCCTGATTTAAAGAAGATTAAGCGAAGTAATCTGAAAGA
TAATTTTCAAGATATTATAGATCAACTCAAAAAAGATCCTTATAAAAAGAATCAAGGATTTGAAAAATTAGAACCACCAA
TTTTAGGAAAATATTCTCGAAGAATCAATATAAAACATCGAGTTGTTTATACTGTTGATGATGTTAATAAGATAGTAAGT
ATTTACTCGGCCTGGTCACATTATGAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10982.66 Da
• Isoelectric Point: 4.6811

>AT201429 WP_072106018.1 NZ_CP074096:c3838959-3838672 [Salmonella enterica subsp. enterica serovar Typhimurium]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDM

Nucleotide

• Length: 288 bp

>AT201429 NZ_CP098403:c738113-737847 [Lactobacillus kefiranofaciens subsp. kefirgranum]
ATGGATGCAACAATTTATACTCCCACAGGAGCAAGACGTAATTTATTCAATCTTATCAAGCAAGTTAATAAAAACCATAC
TCCAGTTAATATTGAATCTACAAAAGATAAAGCAGAAAGTGCTGTGTTAATAAGTAAATCCGACTGGGACGCTATTCAGG
AGACTCTTTATCTTGAGTCCAATGGTGTAGGAGAAGTTGTGAGAAAACGTGAAAAGGACAATTCAGGTTTTACTGATATT
GATGATATTAACTGGGATGAGCTATGA

Structures

Toxin

• AlphaFold DB: A0A5H3Y7E3

Antitoxin

• AlphaFold DB: A0A3Z2K6C6