Detailed information of TA system TA201411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 20096..20621
• Replicon: plasmid pSLT
• Accession: NZ_CP074095
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain A_cip

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: KFU99_RS23590
• Protein ID: WP_001159863.1
• Coordinates: 20316..20621 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: KFU99_RS23585
• Protein ID: WP_000813641.1
• Coordinates: 20096..20314 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU99_RS23560 (KFU99_23565)	15383..15764	+	382	Protein_20	YgiW/YdeI family stress tolerance OB fold protein	-
KFU99_RS23565 (KFU99_23570)	15933..16377	+	445	Protein_21	tyrosine-type recombinase/integrase	-
KFU99_RS23570 (KFU99_23575)	16636..17625	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
KFU99_RS23575 (KFU99_23580)	18119..18415	-	297	WP_001687482.1	hypothetical protein	-
KFU99_RS24205	18427..18855	+	429	Protein_24	hypothetical protein	-
KFU99_RS23580 (KFU99_23585)	18899..19420	-	522	WP_010999942.1	hypothetical protein	-
KFU99_RS23585 (KFU99_23590)	20096..20314	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KFU99_RS23590 (KFU99_23595)	20316..20621	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
KFU99_RS23595 (KFU99_23600)	20623..20913	+	291	WP_001266176.1	hypothetical protein	-
KFU99_RS23600 (KFU99_23605)	20910..21431	+	522	WP_000198608.1	hypothetical protein	-
KFU99_RS23605 (KFU99_23610)	21466..22248	+	783	WP_000082169.1	site-specific integrase	-
KFU99_RS23610 (KFU99_23615)	22257..22808	+	552	WP_000545754.1	EAL domain-containing protein	-
KFU99_RS23615 (KFU99_23620)	22995..23483	+	489	WP_001075507.1	CaiF/GrlA family transcriptional regulator	-
KFU99_RS23620 (KFU99_23625)	23477..23962	+	486	WP_000905606.1	membrane protein	-
KFU99_RS23625 (KFU99_23630)	24239..24526	-	288	WP_071530243.1	hypothetical protein	-
KFU99_RS23630 (KFU99_23635)	24682..25242	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rck / pefD / pefC / pefA / pefB / fdeC / spvC / spvB	1..93933	93933
	-	flank	IS/Tn	-	-	25309..25659	350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11587.48 Da
• Isoelectric Point: 6.9787

>T201411 WP_001159863.1 NZ_CP074095:20316-20621 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Nucleotide

• Length: 306 bp

>T201411 NZ_CP098354:c72359-72210 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8314.16 Da
• Isoelectric Point: 4.5203

>AT201411 WP_000813641.1 NZ_CP074095:20096-20314 [Salmonella enterica subsp. enterica serovar Typhimurium]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Nucleotide

• Length: 219 bp

>AT201411 NZ_CP098354:72403-72464 [Klebsiella pneumoniae]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6