Detailed information of TA system TA201386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4785667..4786243
• Replicon: chromosome
• Accession: NZ_CP074092
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SO_8752_Stm

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: KFK83_RS23095
• Protein ID: WP_001131963.1
• Coordinates: 4785667..4785954 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: KFK83_RS23100
• Protein ID: WP_000063142.1
• Coordinates: 4785941..4786243 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFK83_RS23085 (4780777)	4780777..4784286	-	3510	WP_001043485.1	type I restriction-modification system endonuclease	-
KFK83_RS23090 (4784484)	4784484..4785398	+	915	WP_000290512.1	restriction endonuclease	-
KFK83_RS23095 (4785667)	4785667..4785954	+	288	WP_001131963.1	BrnT family toxin	Toxin
KFK83_RS23100 (4785941)	4785941..4786243	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
KFK83_RS23105 (4786318)	4786318..4787274	-	957	WP_000187839.1	GTPase	-
KFK83_RS23110 (4787285)	4787285..4787488	-	204	WP_000460616.1	YbdD/YjiX family protein	-
KFK83_RS23115 (4787583)	4787583..4789733	-	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T201386 WP_001131963.1 NZ_CP074092:4785667-4785954 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T201386 NZ_CP098350:736776-737261 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT201386 WP_000063142.1 NZ_CP074092:4785941-4786243 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT201386 NZ_CP098350:736486-736779 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01