Detailed information of TA system TA201366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 528134..528754
• Replicon: chromosome
• Accession: NZ_CP074092
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SO_8752_Stm

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: KFK83_RS02390
• Protein ID: WP_001280991.1
• Coordinates: 528134..528352 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: KFK83_RS02395
• Protein ID: WP_000344807.1
• Coordinates: 528380..528754 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFK83_RS02350 (523358)	523358..523927	+	570	WP_000779803.1	YbaY family lipoprotein	-
KFK83_RS02355 (523960)	523960..524349	-	390	WP_000961285.1	MGMT family protein	-
KFK83_RS02365 (524580)	524580..526130	-	1551	WP_000213139.1	EAL domain-containing protein	-
KFK83_RS02370 (526355)	526355..526615	+	261	WP_000801415.1	type B 50S ribosomal protein L31	-
KFK83_RS02375 (526621)	526621..526761	+	141	WP_001197749.1	type B 50S ribosomal protein L36	-
KFK83_RS02380 (526817)	526817..527287	-	471	WP_000136181.1	YlaC family protein	-
KFK83_RS02385 (527404)	527404..527955	-	552	WP_001278792.1	maltose O-acetyltransferase	-
KFK83_RS02390 (528134)	528134..528352	-	219	WP_001280991.1	HHA domain-containing protein	Toxin
KFK83_RS02395 (528380)	528380..528754	-	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
KFK83_RS02400 (529250)	529250..532399	-	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
KFK83_RS02405 (532422)	532422..533615	-	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T201366 WP_001280991.1 NZ_CP074092:c528352-528134 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T201366 NZ_CP098346:55121-55603 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT201366 WP_000344807.1 NZ_CP074092:c528754-528380 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT201366 NZ_CP098346:54867-55133 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0