Detailed information of TA system TA201360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 155216..155886
• Replicon: plasmid pMR0819-ARMA
• Accession: NZ_CP074088
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain MRSN752165

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KF579_RS26930
• Protein ID: WP_004213072.1
• Coordinates: 155216..155659 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KF579_RS26935
• Protein ID: WP_004213073.1
• Coordinates: 155656..155886 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF579_RS26895 (KF579_26900)	150623..150898	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
KF579_RS26900 (KF579_26905)	150961..151452	+	492	WP_011251327.1	DM13 domain-containing protein	-
KF579_RS26905 (KF579_26910)	151501..152421	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
KF579_RS26910 (KF579_26915)	152512..152915	+	404	Protein_171	GAF domain-containing protein	-
KF579_RS26915 (KF579_26920)	153434..154072	-	639	Protein_172	mucoid phenotype regulator RmpA2	-
KF579_RS26920 (KF579_26925)	154489..154793	+	305	Protein_173	transposase	-
KF579_RS29015	154816..155067	-	252	WP_186987481.1	hypothetical protein	-
KF579_RS26930 (KF579_26935)	155216..155659	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KF579_RS26935 (KF579_26940)	155656..155886	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KF579_RS26940 (KF579_26945)	156494..157627	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KF579_RS26945 (KF579_26950)	157643..157936	+	294	WP_004213076.1	hypothetical protein	-
KF579_RS26950 (KF579_26955)	157926..158132	-	207	WP_004213077.1	hypothetical protein	-
KF579_RS26955 (KF579_26960)	158484..158774	+	291	WP_004213078.1	hypothetical protein	-
KF579_RS26960 (KF579_26965)	158764..159663	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..335489	335489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T201360 WP_004213072.1 NZ_CP074088:c155659-155216 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T201360 NZ_CP098343:4263841-4264059 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT201360 WP_004213073.1 NZ_CP074088:c155886-155656 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT201360 NZ_CP098343:4263440-4263814 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620