Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5282617..5283242 Replicon chromosome
Accession NZ_CP074087
Organism Klebsiella pneumoniae subsp. pneumoniae strain MRSN752165

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KF579_RS25655 Protein ID WP_002882817.1
Coordinates 5282617..5283000 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KF579_RS25660 Protein ID WP_004150355.1
Coordinates 5283000..5283242 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF579_RS25640 (5279983) 5279983..5280885 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KF579_RS25645 (5280882) 5280882..5281517 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KF579_RS25650 (5281514) 5281514..5282443 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KF579_RS25655 (5282617) 5282617..5283000 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KF579_RS25660 (5283000) 5283000..5283242 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KF579_RS25665 (5283447) 5283447..5284364 + 918 WP_021466676.1 alpha/beta hydrolase -
KF579_RS25670 (5284378) 5284378..5285319 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KF579_RS25675 (5285364) 5285364..5285801 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KF579_RS25680 (5285798) 5285798..5286658 - 861 WP_004146232.1 virulence factor BrkB family protein -
KF579_RS25685 (5286652) 5286652..5287251 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T201358 WP_002882817.1 NZ_CP074087:c5283000-5282617 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T201358 NZ_CP098343:2055236-2055338 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT201358 WP_004150355.1 NZ_CP074087:c5283242-5283000 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT201358 NZ_CP098343:c2055345-2055200 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References