Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1451960..1452603 Replicon chromosome
Accession NZ_CP074087
Organism Klebsiella pneumoniae subsp. pneumoniae strain MRSN752165

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag KF579_RS07160 Protein ID WP_032433387.1
Coordinates 1452187..1452603 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KF579_RS07155 Protein ID WP_001261276.1
Coordinates 1451960..1452190 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF579_RS07140 (1446982) 1446982..1448069 + 1088 Protein_1396 transcriptional regulator -
KF579_RS07145 (1448072) 1448072..1450312 + 2241 WP_045326788.1 P-loop NTPase fold protein -
KF579_RS07150 (1450840) 1450840..1451655 - 816 WP_032433388.1 hypothetical protein -
KF579_RS07155 (1451960) 1451960..1452190 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KF579_RS07160 (1452187) 1452187..1452603 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
KF579_RS07165 (1452759) 1452759..1453739 + 981 WP_032433385.1 hypothetical protein -
KF579_RS07170 (1453934) 1453934..1455505 - 1572 WP_032433383.1 AAA family ATPase -
KF579_RS07175 (1455824) 1455824..1456072 + 249 WP_032433382.1 hypothetical protein -
KF579_RS07180 (1456131) 1456131..1456649 + 519 WP_045326794.1 hypothetical protein -
KF579_RS07185 (1456680) 1456680..1457171 + 492 WP_032433378.1 hypothetical protein -
KF579_RS07190 (1457231) 1457231..1457434 + 204 WP_032433376.1 HHA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1436596..1482613 46017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T201343 WP_032433387.1 NZ_CP074087:1452187-1452603 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T201343 NZ_CP098340:353038-353385 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT201343 WP_001261276.1 NZ_CP074087:1451960-1452190 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT201343 NZ_CP098340:353385-353684 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References