Detailed information of TA system TA201330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4555227..4555822
• Replicon: chromosome
• Accession: NZ_CP074052
• Organism: Escherichia coli strain PD23

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: KFU85_RS21865
• Protein ID: WP_000239581.1
• Coordinates: 4555227..4555577 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: KFU85_RS21870
• Protein ID: WP_001223213.1
• Coordinates: 4555571..4555822 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU85_RS21845 (4550681)	4550681..4551703	-	1023	WP_001298067.1	ABC transporter permease	-
KFU85_RS21850 (4551717)	4551717..4553219	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
KFU85_RS21855 (4553352)	4553352..4554308	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
KFU85_RS21860 (4554618)	4554618..4555148	+	531	WP_000055075.1	inorganic diphosphatase	-
KFU85_RS21865 (4555227)	4555227..4555577	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
KFU85_RS21870 (4555571)	4555571..4555822	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
KFU85_RS21875 (4556034)	4556034..4556375	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
KFU85_RS21880 (4556378)	4556378..4560157	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T201330 WP_000239581.1 NZ_CP074052:c4555577-4555227 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T201330 NZ_CP098338:350588-350935 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT201330 WP_001223213.1 NZ_CP074052:c4555822-4555571 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT201330 NZ_CP098338:350935-351234 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7