Detailed information of TA system TA020133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 480090..480708
• Replicon: chromosome
• Accession: NC_000913
• Organism: Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: P0ACE3
• Locus tag: b0460
• Protein ID: NP_414993.1
• Coordinates: 480090..480308 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: P0AAR0
• Locus tag: b0461
• Protein ID: NP_414994.1
• Coordinates: 480334..480708 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
b0453	475379..475951	+	573	NP_414987.3	PF09619 family lipoprotein YbaY	-
b0454	475982..476371	-	390	NP_414988.1	DNA base-flipping protein	-
b0456	476672..477025	+	354	NP_414989.1	DUF1428 domain-containing protein YbaA	-
b0457	477067..478617	-	1551	NP_414990.2	c-di-GMP phosphodiesterase PdeB	-
b0458	478781..479251	-	471	NP_414991.4	putative inner membrane protein	-
b0459	479367..479918	-	552	NP_414992.1	maltose O-acetyltransferase	-
b0460	480090..480308	-	219	NP_414993.1	hemolysin expression-modulating protein Hha	Toxin
b0461	480334..480708	-	375	NP_414994.1	Hha toxicity modulator TomB	Antitoxin
b0462	481254..484403	-	3150	NP_414995.1	multidrug efflux pump RND permease AcrB	-
b0463	484426..485619	-	1194	NP_414996.1	multidrug efflux pump membrane fusion lipoprotein AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T20133 NP_414993.1 NC_000913:c480308-480090 [Escherichia coli str. K-12 substr. MG1655]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T20133 NC_000913:c480308-480090 [Escherichia coli str. K-12 substr. MG1655]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT20133 NP_414994.1 NC_000913:c480708-480334 [Escherichia coli str. K-12 substr. MG1655]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT20133 NC_000913:c480708-480334 [Escherichia coli str. K-12 substr. MG1655]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 2MW2
• PDB: 1JW2

Antitoxin

• AlphaFold DB: P0AAR0