Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4266990..4267540 Replicon chromosome
Accession NZ_CP074052
Organism Escherichia coli strain PD23

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag KFU85_RS20605 Protein ID WP_001160966.1
Coordinates 4266990..4267304 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag KFU85_RS20610 Protein ID WP_000125568.1
Coordinates 4267307..4267540 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU85_RS20580 (4262665) 4262665..4263951 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KFU85_RS20585 (4263951) 4263951..4264940 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KFU85_RS20590 (4264937) 4264937..4265758 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KFU85_RS20595 (4265761) 4265761..4266138 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KFU85_RS20600 (4266145) 4266145..4266993 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KFU85_RS20605 (4266990) 4266990..4267304 - 315 WP_001160966.1 CcdB family protein Toxin
KFU85_RS20610 (4267307) 4267307..4267540 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KFU85_RS20615 (4267626) 4267626..4268105 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
KFU85_RS20620 (4268297) 4268297..4270159 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
KFU85_RS20625 (4270152) 4270152..4270682 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KFU85_RS20630 (4270790) 4270790..4272121 - 1332 WP_001183201.1 MFS transporter -
KFU85_RS20635 (4272179) 4272179..4272466 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T201328 WP_001160966.1 NZ_CP074052:c4267304-4266990 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T201328 NZ_CP098337:7141-7557 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT201328 WP_000125568.1 NZ_CP074052:c4267540-4267307 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT201328 NZ_CP098337:6914-7144 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References