Detailed information of TA system TA201324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3390447..3391152
• Replicon: chromosome
• Accession: NZ_CP074052
• Organism: Escherichia coli strain PD23

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: KFU85_RS16495
• Protein ID: WP_000539521.1
• Coordinates: 3390447..3390833 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KFU85_RS16500
• Protein ID: WP_001280945.1
• Coordinates: 3390823..3391152 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU85_RS16475 (3386451)	3386451..3387077	+	627	WP_001295292.1	glutathione S-transferase GstB	-
KFU85_RS16480 (3387074)	3387074..3388189	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
KFU85_RS16485 (3388300)	3388300..3388683	-	384	WP_000497137.1	biofilm formation regulator BssR	-
KFU85_RS16490 (3388896)	3388896..3390221	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KFU85_RS16495 (3390447)	3390447..3390833	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU85_RS16500 (3390823)	3390823..3391152	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
KFU85_RS16505 (3391222)	3391222..3392550	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
KFU85_RS16510 (3392558)	3392558..3394906	-	2349	WP_022645413.1	EAL domain-containing protein	-
KFU85_RS16515 (3395083)	3395083..3395994	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T201324 WP_000539521.1 NZ_CP074052:3390447-3390833 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T201324 NZ_CP098335:c4909128-4908844 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT201324 WP_001280945.1 NZ_CP074052:3390823-3391152 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT201324 NZ_CP098335:c4909360-4909118 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6