Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 952096..952750 Replicon chromosome
Accession NZ_CP074052
Organism Escherichia coli strain PD23

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KFU85_RS04555 Protein ID WP_000244781.1
Coordinates 952343..952750 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU85_RS04550 Protein ID WP_000354046.1
Coordinates 952096..952362 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU85_RS04525 (947387) 947387..948136 + 750 WP_209319277.1 transporter -
KFU85_RS04530 (948184) 948184..949617 - 1434 WP_136698981.1 6-phospho-beta-glucosidase BglA -
KFU85_RS04535 (949662) 949662..949973 + 312 WP_077516667.1 N(4)-acetylcytidine aminohydrolase -
KFU85_RS04540 (950137) 950137..950796 + 660 WP_000250269.1 hemolysin III family protein -
KFU85_RS04545 (950873) 950873..951853 - 981 WP_077516665.1 tRNA-modifying protein YgfZ -
KFU85_RS04550 (952096) 952096..952362 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU85_RS04555 (952343) 952343..952750 + 408 WP_000244781.1 protein YgfX Toxin
KFU85_RS04560 (952790) 952790..953311 - 522 WP_032303736.1 flavodoxin FldB -
KFU85_RS04565 (953423) 953423..954319 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
KFU85_RS04570 (954344) 954344..955054 + 711 WP_077516663.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU85_RS04575 (955060) 955060..956793 + 1734 WP_077516661.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T201310 WP_000244781.1 NZ_CP074052:952343-952750 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201310 NZ_CP098330:c4599276-4598992 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201310 WP_000354046.1 NZ_CP074052:952096-952362 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201310 NZ_CP098330:c4599508-4599266 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References