Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 166370..166982 Replicon chromosome
Accession NZ_CP074052
Organism Escherichia coli strain PD23

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag KFU85_RS00725 Protein ID WP_000833473.1
Coordinates 166370..166555 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag KFU85_RS00730 Protein ID WP_022646255.1
Coordinates 166572..166982 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU85_RS00710 (161836) 161836..163131 + 1296 WP_000985736.1 Fic family protein -
KFU85_RS00715 (163239) 163239..164777 + 1539 WP_000183976.1 aldehyde dehydrogenase AldB -
KFU85_RS00720 (164818) 164818..165897 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
KFU85_RS00725 (166370) 166370..166555 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU85_RS00730 (166572) 166572..166982 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU85_RS00735 (167103) 167103..169073 - 1971 WP_077517025.1 glycoside hydrolase family 127 protein -
KFU85_RS00740 (169084) 169084..170484 - 1401 WP_000204814.1 MFS transporter -
KFU85_RS00745 (170710) 170710..171525 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T201307 WP_000833473.1 NZ_CP074052:166370-166555 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T201307 NZ_CP098330:2141639-2141742 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT201307 WP_022646255.1 NZ_CP074052:166572-166982 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT201307 NZ_CP098330:c2141747-2141604 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References