Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3624302..3624969 | Replicon | chromosome |
| Accession | NZ_CP074047 | ||
| Organism | Escherichia coli strain PD7 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | A0A1Z3MRP6 |
| Locus tag | KFU78_RS17500 | Protein ID | WP_001581456.1 |
| Coordinates | 3624640..3624969 (+) | Length | 110 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | S1EWR7 |
| Locus tag | KFU78_RS17495 | Protein ID | WP_000070396.1 |
| Coordinates | 3624302..3624619 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFU78_RS17450 (3619739) | 3619739..3620560 | + | 822 | WP_001581463.1 | DUF932 domain-containing protein | - |
| KFU78_RS17455 (3620777) | 3620777..3621478 | + | 702 | WP_001581462.1 | WYL domain-containing protein | - |
| KFU78_RS17460 (3621519) | 3621519..3621755 | + | 237 | WP_001144031.1 | protein YpjK | - |
| KFU78_RS17465 (3621755) | 3621755..3622198 | + | 444 | WP_001581461.1 | lipoprotein YafY | - |
| KFU78_RS17470 (3622222) | 3622222..3622689 | + | 468 | WP_001581459.1 | protein YkfB | - |
| KFU78_RS17475 (3622766) | 3622766..3623005 | + | 240 | WP_000194654.1 | DUF905 family protein | - |
| KFU78_RS17480 (3623103) | 3623103..3623561 | + | 459 | WP_000211838.1 | antirestriction protein | - |
| KFU78_RS17485 (3623577) | 3623577..3624053 | + | 477 | WP_001581457.1 | RadC family protein | - |
| KFU78_RS17490 (3624062) | 3624062..3624283 | + | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| KFU78_RS17495 (3624302) | 3624302..3624619 | + | 318 | WP_000070396.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| KFU78_RS17500 (3624640) | 3624640..3624969 | + | 330 | WP_001581456.1 | toxin | Toxin |
| KFU78_RS17505 (3625320) | 3625320..3626177 | - | 858 | WP_001581455.1 | DeoR/GlpR family DNA-binding transcription regulator | - |
| KFU78_RS17510 (3626308) | 3626308..3627258 | - | 951 | WP_001581454.1 | D-ribose ABC transporter substrate-binding protein | - |
| KFU78_RS17515 (3627347) | 3627347..3628408 | - | 1062 | WP_001581452.1 | ABC transporter permease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | gmhA/lpcA / hcp1/tssD1 | 3552689..3724152 | 171463 | |
| - | inside | Prophage | - | gmhA/lpcA / hcp1/tssD1 | 3617651..3724152 | 106501 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 110 a.a. Molecular weight: 12456.52 Da Isoelectric Point: 8.5011
>T201301 WP_001581456.1 NZ_CP074047:3624640-3624969 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK
Download Length: 330 bp
>T201301 NZ_CP098325:c3834856-3834443 [Leclercia adecarboxylata]
GTGGTCCTGTGGCAATCTGATCTACGCGTCTCGTGGCGCTCCCAGTGGATGTCTCTTCTGCTCCACGGCCTGGTCGCGGC
TTTCATTCTGCTGATGCCGTGGCCGCTGAGCTACACCCCGTTATGGCTAATACTGCTGTCGCTGGTGGTGTTTGATAGCG
TCCGCAGCCAGCGGCGCATTAACGCCCGCCAGGGTGAAATCAAACTGTTCATGGATTCCCGCCTGCACTGGCTGGGCAGC
GAGTGGGAGATTGTCGGCACGCCGTGGATGCTCAATTCCGGCATGATGCTGCGTTTGCGCAAAAGTGCAGGGCAACGCTG
CCATCACCTGTGGCTGGCGGCAGACAGCATGGACGAAGGGGAGTGGCGGGATCTGCGTCGGATGATCTCACACCAGCCGA
CGCAGGGGCGGTAG
GTGGTCCTGTGGCAATCTGATCTACGCGTCTCGTGGCGCTCCCAGTGGATGTCTCTTCTGCTCCACGGCCTGGTCGCGGC
TTTCATTCTGCTGATGCCGTGGCCGCTGAGCTACACCCCGTTATGGCTAATACTGCTGTCGCTGGTGGTGTTTGATAGCG
TCCGCAGCCAGCGGCGCATTAACGCCCGCCAGGGTGAAATCAAACTGTTCATGGATTCCCGCCTGCACTGGCTGGGCAGC
GAGTGGGAGATTGTCGGCACGCCGTGGATGCTCAATTCCGGCATGATGCTGCGTTTGCGCAAAAGTGCAGGGCAACGCTG
CCATCACCTGTGGCTGGCGGCAGACAGCATGGACGAAGGGGAGTGGCGGGATCTGCGTCGGATGATCTCACACCAGCCGA
CGCAGGGGCGGTAG
Antitoxin
Download Length: 106 a.a. Molecular weight: 11982.62 Da Isoelectric Point: 7.0270
>AT201301 WP_000070396.1 NZ_CP074047:3624302-3624619 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT201301 NZ_CP098325:c3835103-3834837 [Leclercia adecarboxylata]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGTCGCGGCATGCGCGAACTCGATATCTCCATCATGCCGTT
CTTCGAATACGAATATGACAGCCTGAGCGATGAGGATAAGCAGCTCTTTATCCGCCTGCTGGAGAACGACGATCCTGACT
TATTTAACTGGCTGATGAATCACGGCGAACCCGCCGACACCGAACTGCAACGGATGGTGCTTTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGTCGCGGCATGCGCGAACTCGATATCTCCATCATGCCGTT
CTTCGAATACGAATATGACAGCCTGAGCGATGAGGATAAGCAGCTCTTTATCCGCCTGCTGGAGAACGACGATCCTGACT
TATTTAACTGGCTGATGAATCACGGCGAACCCGCCGACACCGAACTGCAACGGATGGTGCTTTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Z3MRP6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1EWR7 |