Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3624302..3624969 Replicon chromosome
Accession NZ_CP074047
Organism Escherichia coli strain PD7

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3MRP6
Locus tag KFU78_RS17500 Protein ID WP_001581456.1
Coordinates 3624640..3624969 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag KFU78_RS17495 Protein ID WP_000070396.1
Coordinates 3624302..3624619 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU78_RS17450 (3619739) 3619739..3620560 + 822 WP_001581463.1 DUF932 domain-containing protein -
KFU78_RS17455 (3620777) 3620777..3621478 + 702 WP_001581462.1 WYL domain-containing protein -
KFU78_RS17460 (3621519) 3621519..3621755 + 237 WP_001144031.1 protein YpjK -
KFU78_RS17465 (3621755) 3621755..3622198 + 444 WP_001581461.1 lipoprotein YafY -
KFU78_RS17470 (3622222) 3622222..3622689 + 468 WP_001581459.1 protein YkfB -
KFU78_RS17475 (3622766) 3622766..3623005 + 240 WP_000194654.1 DUF905 family protein -
KFU78_RS17480 (3623103) 3623103..3623561 + 459 WP_000211838.1 antirestriction protein -
KFU78_RS17485 (3623577) 3623577..3624053 + 477 WP_001581457.1 RadC family protein -
KFU78_RS17490 (3624062) 3624062..3624283 + 222 WP_000691994.1 DUF987 domain-containing protein -
KFU78_RS17495 (3624302) 3624302..3624619 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
KFU78_RS17500 (3624640) 3624640..3624969 + 330 WP_001581456.1 toxin Toxin
KFU78_RS17505 (3625320) 3625320..3626177 - 858 WP_001581455.1 DeoR/GlpR family DNA-binding transcription regulator -
KFU78_RS17510 (3626308) 3626308..3627258 - 951 WP_001581454.1 D-ribose ABC transporter substrate-binding protein -
KFU78_RS17515 (3627347) 3627347..3628408 - 1062 WP_001581452.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / hcp1/tssD1 3552689..3724152 171463
- inside Prophage - gmhA/lpcA / hcp1/tssD1 3617651..3724152 106501


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12456.52 Da        Isoelectric Point: 8.5011

>T201301 WP_001581456.1 NZ_CP074047:3624640-3624969 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK

Download         Length: 330 bp

>T201301 NZ_CP098325:c3834856-3834443 [Leclercia adecarboxylata]
GTGGTCCTGTGGCAATCTGATCTACGCGTCTCGTGGCGCTCCCAGTGGATGTCTCTTCTGCTCCACGGCCTGGTCGCGGC
TTTCATTCTGCTGATGCCGTGGCCGCTGAGCTACACCCCGTTATGGCTAATACTGCTGTCGCTGGTGGTGTTTGATAGCG
TCCGCAGCCAGCGGCGCATTAACGCCCGCCAGGGTGAAATCAAACTGTTCATGGATTCCCGCCTGCACTGGCTGGGCAGC
GAGTGGGAGATTGTCGGCACGCCGTGGATGCTCAATTCCGGCATGATGCTGCGTTTGCGCAAAAGTGCAGGGCAACGCTG
CCATCACCTGTGGCTGGCGGCAGACAGCATGGACGAAGGGGAGTGGCGGGATCTGCGTCGGATGATCTCACACCAGCCGA
CGCAGGGGCGGTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT201301 WP_000070396.1 NZ_CP074047:3624302-3624619 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT201301 NZ_CP098325:c3835103-3834837 [Leclercia adecarboxylata]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGTCGCGGCATGCGCGAACTCGATATCTCCATCATGCCGTT
CTTCGAATACGAATATGACAGCCTGAGCGATGAGGATAAGCAGCTCTTTATCCGCCTGCTGGAGAACGACGATCCTGACT
TATTTAACTGGCTGATGAATCACGGCGAACCCGCCGACACCGAACTGCAACGGATGGTGCTTTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3MRP6

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References