Detailed information of TA system TA201299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2395971..2396609
• Replicon: chromosome
• Accession: NZ_CP074047
• Organism: Escherichia coli strain PD7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU78_RS11490
• Protein ID: WP_000813794.1
• Coordinates: 2396433..2396609 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU78_RS11485
• Protein ID: WP_001270286.1
• Coordinates: 2395971..2396387 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU78_RS11465 (2391123)	2391123..2392064	-	942	WP_001251311.1	ABC transporter permease	-
KFU78_RS11470 (2392065)	2392065..2393078	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
KFU78_RS11475 (2393096)	2393096..2394241	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KFU78_RS11480 (2394486)	2394486..2395892	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
KFU78_RS11485 (2395971)	2395971..2396387	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU78_RS11490 (2396433)	2396433..2396609	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU78_RS11495 (2396831)	2396831..2397061	+	231	WP_000494244.1	YncJ family protein	-
KFU78_RS11500 (2397153)	2397153..2399114	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU78_RS11505 (2399187)	2399187..2399723	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
KFU78_RS11510 (2399776)	2399776..2400990	+	1215	WP_071597387.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2401030..2402295	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T201299 WP_000813794.1 NZ_CP074047:c2396609-2396433 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T201299 NZ_CP098325:c2456742-2456488 [Leclercia adecarboxylata]
ATGGTACGAGCTGGACTGACGGCAGTAACCCATTCTGCATTGATTGGCGCAGCCCGGATGGGATTTTGCTCCAGACAGGA
GATTTTCGATGTTGTCCTGGCACTGGAACCCACCGACTTTTACAAAAGTATGACGGCTCATCACGACCATACGTGCTGGC
ACGACGTGTATCGGACTGTGTATAAAGACCAGACAATTTACATGAAACTTATCGTGACTGATGGTGTGCTGGTGGTGTCA
TTTAAGGAGTTATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT201299 WP_001270286.1 NZ_CP074047:c2396387-2395971 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT201299 NZ_CP098325:c2456491-2456084 [Leclercia adecarboxylata]
ATGAAAATGAAATGTCCTGTCTGCGGTGGCGCGGCGTTGAAGCATGAAAGCCGCGATGTTGCTTATGAATACAAAGGCCA
AAAAACAATTTTTCATGCCGTTAAGGGGCAGTTTTGCGATGCGTGCGGCGAAATTATTTTCGCGAACGGGGAAGGGGATG
ATTACTTTGCCGCCATGCTGGAGTTTAACCGCAAAGTGAATGCCGAAGAGGTCGATCCCACTTTCATCGCTAAGGTGCGA
AAGCGCCTGAATCTGGATCAGCGTCAGGCGGCAGAAATTTTTGGCGGCGGGGTCAATGCCTTTAGTCGTTACGAAACGGG
GCGTGTAGCACCGCCAAAACCGTTAGTGCTGTTGTTTAAAGCACTGGATAAGCACCCTGAACTTTTAGATGAGCTGACGC
AGGCTTAA

Structures

Toxin

• PDB: 6HPB