Detailed information of TA system TA201282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 45058..45856
• Replicon: plasmid pYLPI6a
• Accession: NZ_CP074043
• Organism: Escherichia coli strain PI6

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KFU63_RS22515
• Protein ID: WP_033870689.1
• Coordinates: 45335..45856 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: KFU63_RS22510
• Protein ID: WP_001711191.1
• Coordinates: 45058..45327 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU63_RS22485 (KFU63_22300)	40482..41822	-	1341	WP_023135655.1	DnaB-like helicase C-terminal domain-containing protein	-
KFU63_RS22490 (KFU63_22305)	41883..42608	-	726	WP_023135656.1	hypothetical protein	-
KFU63_RS22495 (KFU63_22310)	42891..43658	+	768	WP_033870691.1	hypothetical protein	-
KFU63_RS22500 (KFU63_22315)	43711..44064	-	354	WP_160378290.1	hypothetical protein	-
KFU63_RS22505 (KFU63_22320)	44070..44738	-	669	WP_000161228.1	division plane positioning ATPase MipZ	-
KFU63_RS22510 (KFU63_22325)	45058..45327	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
KFU63_RS22515 (KFU63_22330)	45335..45856	+	522	WP_033870689.1	GNAT family N-acetyltransferase	Toxin
KFU63_RS22520 (KFU63_22335)	46025..46276	-	252	WP_023135660.1	hypothetical protein	-
KFU63_RS22525 (KFU63_22340)	46279..46971	-	693	WP_033546097.1	membrane protein	-
KFU63_RS22530 (KFU63_22345)	46985..47308	-	324	WP_001711185.1	hypothetical protein	-
KFU63_RS22535 (KFU63_22350)	47383..48168	-	786	WP_257182276.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96383	96383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19395.22 Da
• Isoelectric Point: 8.2535

>T201282 WP_033870689.1 NZ_CP074043:45335-45856 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKENLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T201282 NZ_CP098257:2268673-2268990 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATATGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAATTAGAAAAAGCATCATCTGAGAACTTTGACGAGGAATCAGATGACGCGCAGAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT201282 WP_001711191.1 NZ_CP074043:45058-45327 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT201282 NZ_CP098257:2268461-2268670 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2