Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3436859..3437696 Replicon chromosome
Accession NZ_CP074042
Organism Escherichia coli strain PI6

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag KFU63_RS16560 Protein ID WP_000227784.1
Coordinates 3437154..3437696 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag KFU63_RS16555 Protein ID WP_001297137.1
Coordinates 3436859..3437170 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU63_RS16530 (3431879) 3431879..3432826 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
KFU63_RS16535 (3432848) 3432848..3434839 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KFU63_RS16540 (3434829) 3434829..3435443 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KFU63_RS16545 (3435443) 3435443..3435772 + 330 WP_025649657.1 cytochrome o ubiquinol oxidase subunit IV -
KFU63_RS16550 (3435784) 3435784..3436674 + 891 WP_000971336.1 heme o synthase -
KFU63_RS16555 (3436859) 3436859..3437170 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
KFU63_RS16560 (3437154) 3437154..3437696 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
KFU63_RS16565 (3437752) 3437752..3438687 - 936 WP_001297127.1 tetratricopeptide repeat protein -
KFU63_RS16570 (3439095) 3439095..3440459 + 1365 WP_001000978.1 MFS transporter -
KFU63_RS16575 (3440587) 3440587..3441078 - 492 WP_001138904.1 nucleotide binding protein YajQ -
KFU63_RS16580 (3441246) 3441246..3442157 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T201277 WP_000227784.1 NZ_CP074042:3437154-3437696 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T201277 NZ_CP098257:c1176283-1175960 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGA
GTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT201277 WP_001297137.1 NZ_CP074042:3436859-3437170 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT201277 NZ_CP098257:c1176495-1176286 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGTGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References