Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3402781..3403399 Replicon chromosome
Accession NZ_CP074042
Organism Escherichia coli strain PI6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU63_RS16390 Protein ID WP_001291435.1
Coordinates 3403181..3403399 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU63_RS16385 Protein ID WP_000344800.1
Coordinates 3402781..3403155 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU63_RS16375 (3397870) 3397870..3399063 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU63_RS16380 (3399086) 3399086..3402235 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU63_RS16385 (3402781) 3402781..3403155 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU63_RS16390 (3403181) 3403181..3403399 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU63_RS16395 (3403571) 3403571..3404122 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU63_RS16400 (3404238) 3404238..3404708 + 471 WP_000136192.1 YlaC family protein -
KFU63_RS16405 (3404872) 3404872..3406422 + 1551 WP_112044478.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU63_RS16410 (3406464) 3406464..3406817 - 354 WP_000878141.1 DUF1428 family protein -
KFU63_RS16420 (3407196) 3407196..3407507 + 312 WP_000409911.1 MGMT family protein -
KFU63_RS16425 (3407538) 3407538..3408110 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201276 WP_001291435.1 NZ_CP074042:3403181-3403399 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201276 NZ_CP098257:714091-714279 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201276 WP_000344800.1 NZ_CP074042:3402781-3403155 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201276 NZ_CP098257:713802-714062 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References