Detailed information of TA system TA201274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2345554..2347142
• Replicon: chromosome
• Accession: NZ_CP074042
• Organism: Escherichia coli strain PI6

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: KFU63_RS11370
• Protein ID: WP_001125463.1
• Coordinates: 2345820..2347142 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KFU63_RS11365
• Protein ID: WP_001296726.1
• Coordinates: 2345554..2345820 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU63_RS11355 (2340777)	2340777..2342005	+	1229	WP_089541817.1	IS3 family transposase	-
KFU63_RS11360 (2342017)	2342017..2345346	+	3330	Protein_2222	ECSE_1600 family autotransporter	-
KFU63_RS11365 (2345554)	2345554..2345820	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KFU63_RS11370 (2345820)	2345820..2347142	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KFU63_RS11375 (2347454)	2347454..2347662	+	209	Protein_2225	AraC family transcriptional regulator	-
KFU63_RS11380 (2348094)	2348094..2348657	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
KFU63_RS11385 (2349018)	2349018..2349728	+	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2340777..2358349	17572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T201274 WP_001125463.1 NZ_CP074042:2345820-2347142 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T201274 NZ_CP098255:2268673-2268990 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATATGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAATTAGAAAAAGCATCATCTGAGAACTTTGACGAGGAATCAGATGACGCGCAGAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT201274 WP_001296726.1 NZ_CP074042:2345554-2345820 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT201274 NZ_CP098255:2268461-2268670 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0