Detailed information of TA system TA020127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2516150..2516749
• Replicon: chromosome
• Accession: NC_000911
• Organism: Synechocystis sp. PCC 6803

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SGL_RS13570
• Protein ID: WP_010873447.1
• Coordinates: 2516150..2516527 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SGL_RS13575
• Protein ID: WP_010873448.1
• Coordinates: 2516528..2516749 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SGL_RS13555 (sll0208)	2511514..2512209	-	696	WP_010873444.1	aldehyde oxygenase (deformylating)	-
SGL_RS13560 (sll0207)	2512467..2513564	-	1098	WP_014407137.1	glucose-1-phosphate thymidylyltransferase	-
SGL_RS13565 (slr0220)	2513806..2515974	+	2169	WP_010873446.1	glycine--tRNA ligase subunit beta	-
SGL_RS13570 (sll0205)	2516150..2516527	-	378	WP_010873447.1	type II toxin-antitoxin system VapC family toxin	Toxin
SGL_RS13575 (slr1000)	2516528..2516749	-	222	WP_010873448.1	DUF2281 domain-containing protein	Antitoxin
SGL_RS13580 (sll0204)	2516814..2518184	-	1371	WP_010873449.1	FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (2-124)

Antitoxin

• Domain of unknown function DUF2281 (6-72)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14761.18 Da
• Isoelectric Point: 7.0607

>T20127 WP_010873447.1 NC_000911:c2516527-2516150 [Synechocystis sp. PCC 6803]
MLLDTHTLLWFLDNDIKLPSHLKSLIEAKPKVYVSIVSLWEIAIKLNINKLRLNYAFTDLENLLADLNISVLNITWQHLE
ACRNLPLHHRDPFDRMIIAQAQQHNFSVISKDENFKKYNVNLIWE

Nucleotide

• Length: 378 bp

>T20127 NC_000911:c2516527-2516150 [Synechocystis sp. PCC 6803]
ATGCTACTGGACACCCACACGCTTTTGTGGTTTTTAGATAATGATATAAAACTACCATCACATCTCAAGAGTTTGATTGA
AGCCAAGCCAAAAGTTTATGTCAGCATTGTATCTCTCTGGGAGATTGCCATTAAACTCAACATCAACAAACTAAGGCTTA
ATTACGCTTTTACTGATCTAGAGAATTTACTTGCTGATCTCAATATTTCGGTTTTGAACATCACATGGCAACATTTGGAA
GCTTGTCGCAATTTGCCCTTGCATCATCGAGATCCTTTTGACCGTATGATTATTGCCCAGGCTCAGCAACATAATTTTTC
CGTCATTAGCAAAGACGAAAATTTCAAAAAATACAACGTTAATTTAATCTGGGAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8355.66 Da
• Isoelectric Point: 4.3855

>AT20127 WP_010873448.1 NC_000911:c2516749-2516528 [Synechocystis sp. PCC 6803]
MTDTALLEKINALPDAMKIEVEHFVEFLLTKQPPVSPVTNDGQKKYRQAGVLKGKIWMADDFDAPLEEMQEYM

Nucleotide

• Length: 222 bp

>AT20127 NC_000911:c2516749-2516528 [Synechocystis sp. PCC 6803]
ATGACAGACACCGCCCTACTAGAAAAGATCAATGCTCTCCCCGATGCCATGAAAATTGAAGTAGAACACTTTGTCGAATT
TCTGCTGACTAAGCAACCACCAGTCAGTCCAGTAACCAATGATGGACAAAAAAAATATCGTCAAGCAGGTGTTCTAAAGG
GGAAAATTTGGATGGCAGATGACTTTGATGCTCCTTTGGAAGAAATGCAGGAGTATATGTAA