Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1320344..1320969 Replicon chromosome
Accession NZ_CP074042
Organism Escherichia coli strain PI6

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KFU63_RS06385 Protein ID WP_000911329.1
Coordinates 1320571..1320969 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KFU63_RS06380 Protein ID WP_000450524.1
Coordinates 1320344..1320571 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU63_RS06355 (1316146) 1316146..1316616 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KFU63_RS06360 (1316616) 1316616..1317188 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KFU63_RS06365 (1317334) 1317334..1318212 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KFU63_RS06370 (1318229) 1318229..1319263 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KFU63_RS06375 (1319476) 1319476..1320189 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KFU63_RS06380 (1320344) 1320344..1320571 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KFU63_RS06385 (1320571) 1320571..1320969 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU63_RS06390 (1321116) 1321116..1321979 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KFU63_RS06395 (1321994) 1321994..1324009 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
KFU63_RS06400 (1324083) 1324083..1324781 + 699 WP_000679812.1 esterase -
KFU63_RS06405 (1324891) 1324891..1325091 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T201264 WP_000911329.1 NZ_CP074042:1320571-1320969 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T201264 NZ_CP098253:2195347-2195556 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT201264 WP_000450524.1 NZ_CP074042:1320344-1320571 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT201264 NZ_CP098253:2195042-2195335 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References