Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 882851..883505 Replicon chromosome
Accession NZ_CP074042
Organism Escherichia coli strain PI6

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFU63_RS04325 Protein ID WP_000244772.1
Coordinates 883098..883505 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU63_RS04320 Protein ID WP_000354046.1
Coordinates 882851..883117 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU63_RS04295 (878139) 878139..878882 + 744 WP_001436959.1 SDR family oxidoreductase -
KFU63_RS04300 (878939) 878939..880372 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
KFU63_RS04305 (880417) 880417..880728 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFU63_RS04310 (880892) 880892..881551 + 660 WP_000250274.1 hemolysin III family protein -
KFU63_RS04315 (881628) 881628..882608 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU63_RS04320 (882851) 882851..883117 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU63_RS04325 (883098) 883098..883505 + 408 WP_000244772.1 protein YgfX Toxin
KFU63_RS04330 (883545) 883545..884066 - 522 WP_001055874.1 flavodoxin FldB -
KFU63_RS04335 (884178) 884178..885074 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU63_RS04340 (885099) 885099..885809 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU63_RS04345 (885815) 885815..887548 + 1734 WP_000813213.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T201262 WP_000244772.1 NZ_CP074042:883098-883505 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201262 NZ_CP098253:1821319-1821585 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGATCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201262 WP_000354046.1 NZ_CP074042:882851-883117 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201262 NZ_CP098253:1821062-1821319 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References