Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 329033..329833 Replicon chromosome
Accession NZ_CP074042
Organism Escherichia coli strain PI6

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KFU63_RS01540 Protein ID WP_000342449.1
Coordinates 329306..329833 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KFU63_RS01535 Protein ID WP_001277108.1
Coordinates 329033..329299 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU63_RS01515 (324691) 324691..325359 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KFU63_RS01520 (325352) 325352..326410 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KFU63_RS01525 (326655) 326655..327509 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
KFU63_RS01530 (327780) 327780..328883 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KFU63_RS01535 (329033) 329033..329299 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KFU63_RS01540 (329306) 329306..329833 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KFU63_RS01545 (329830) 329830..330213 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
KFU63_RS01550 (330637) 330637..331746 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KFU63_RS01555 (331794) 331794..332720 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KFU63_RS01560 (332717) 332717..333994 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
KFU63_RS01565 (333991) 333991..334758 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T201260 WP_000342449.1 NZ_CP074042:329306-329833 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T201260 NZ_CP098253:714100-714288 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT201260 WP_001277108.1 NZ_CP074042:329033-329299 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT201260 NZ_CP098253:713811-714071 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References