Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 301961..302592 Replicon chromosome
Accession NZ_CP074042
Organism Escherichia coli strain PI6

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag KFU63_RS01380 Protein ID WP_001259385.1
Coordinates 301961..302236 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag KFU63_RS01385 Protein ID WP_000593554.1
Coordinates 302233..302592 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU63_RS01365 (296965) 296965..298032 + 1068 WP_000361467.1 HlyD family secretion protein -
KFU63_RS01370 (298029) 298029..300764 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
KFU63_RS01375 (300764) 300764..301888 + 1125 WP_025650299.1 ABC transporter permease -
KFU63_RS01380 (301961) 301961..302236 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU63_RS01385 (302233) 302233..302592 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU63_RS01390 (302642) 302642..303502 - 861 WP_001131783.1 class II aldolase -
KFU63_RS01395 (303534) 303534..303803 - 270 WP_000933060.1 HPr family phosphocarrier protein -
KFU63_RS01400 (303793) 303793..305301 - 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
KFU63_RS01405 (305294) 305294..306652 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
KFU63_RS01410 (306729) 306729..307010 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
KFU63_RS01415 (307007) 307007..307480 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 262340..302592 40252


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T201259 WP_001259385.1 NZ_CP074042:301961-302236 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T201259 NZ_CP098251:c161721-161443 [Oxalobacter aliiformigenes]
ATGATTCAATCATTTTCATGTGCTGATACGCAAGCGCTTTTTGCTGGCCGTTCTGTTCCGCGATTTGTGAATATTCGCAC
GGTGGCAGAGCGCAAGCTCCAGATGCTTCACAGGGCCGTTTGCCTTGATGATTTGCGTATTCCACCGAATAACCGTCTTG
AGGCGCTCAAGGGGGACAGGAAGGGGCAGTACAGTATCCGGATTAACGGCCAATGGCGGTTGTGTTTCCGGTTTGAAGGG
GGCCATGCGTTTGATGTGGCTATTGTGGATTATCACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT201259 WP_000593554.1 NZ_CP074042:302233-302592 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT201259 NZ_CP098251:c161429-161124 [Oxalobacter aliiformigenes]
ATGGATGAGGTTATTAACGGTATGCGGCCCGTTCATCCGGGTGAAATTTTGCGGGAAGAATTTTTGAAGCCGTTGAATTT
ATCCGCGAATGGTCTGGCGATTGATTTGCATGTCCCGGCACCCCGCATTAACGATATTGTCCGGGAAAAACGGGGGATTA
CGGCAGATACCGCCTTGAGACTGGCCCGGTATTTCGGAACAAGTGCGGAATTCTGGCTGGGTTTGCAGGATGATTACGAT
CTGAAAACCTGTCGAGAACAATTACATGATACGTTAGATCGGATTGTTCCGAGGGCTACTGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References