Detailed information of TA system TA201254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1987..2534
• Replicon: plasmid pYLPI7c
• Accession: NZ_CP074036
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KFU70_RS24940
• Protein ID: WP_244442846.1
• Coordinates: 2256..2534 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: KFU70_RS24935
• Protein ID: WP_000079941.1
• Coordinates: 1987..2256 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS24930 (46)	46..1077	+	1032	WP_000907869.1	plasmid replication initiator RepA	-
KFU70_RS24935 (1987)	1987..2256	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
KFU70_RS24940 (2256)	2256..2534	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
KFU70_RS24945 (2574)	2574..3422	+	849	WP_001057996.1	3'-5' exonuclease	-
KFU70_RS24950 (3563)	3563..4012	+	450	WP_001236003.1	hypothetical protein	-
KFU70_RS24955 (4489)	4489..4755	-	267	WP_000955263.1	hypothetical protein	-
KFU70_RS24960 (4755)	4755..5249	-	495	WP_001459505.1	hypothetical protein	-
KFU70_RS24965 (5305)	5305..5907	-	603	WP_000517695.1	ProQ/FINO family protein	-
KFU70_RS24970 (6278)	6278..6982	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaCTX-M-15	-	1..88772	88772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T201254 WP_244442846.1 NZ_CP074036:2256-2534 [Escherichia coli]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T201254 NZ_CP098247:1007786-1007971 [Oxalobacter aliiformigenes]
ATGGACAGCAAAGAAATCATCAAACAACTCGAAAAGGCCGGATGGAAATTGCGAGACGTCAATGGATCGCATCACATTTA
CACCCATCCTGAACGGGGCGGTCATATTTCAATTCCTCATCCCAAAAAGGATCTGGGTGTCGGTCTTGTCAAAAAGTTGC
TTAAACAGGCTGGTTTAAAGGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT201254 WP_000079941.1 NZ_CP074036:1987-2256 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT201254 NZ_CP098247:1007976-1008362 [Oxalobacter aliiformigenes]
ATGAAATATCCCATTGCGATTGAACCGGGTACTGACGATACCGCTTTCGGTGTCGTGGTTCCTGATCTTCCTGGTTGCTT
TTCGGCGGGTGACACTTTGGAAGAAGCGATTGACAATGCCGGTGAAGCAGCCAAATTGTGGCTGGAAACGGTTATCGATG
ATGGTGGAATAGTTCCTGCCGCCTCGTCATTTTCGGTATTGCAGACAAAAGACGATTACAAGGGCTGGATTTGGGCAGTC
ATCGATGTTGATCTTTCGGATTTGTCCGACAAAACAGAACGCATCAATATCACATTGCCATCCAGAGTGTTAAGACGCAT
TGACCGGGATGCAAAAGCTGCCGGCGAAAGTCGTTCCGGTTTCATCGCCAGAAGGATGTTGTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5