Detailed information of TA system TA201253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 1803..2404
• Replicon: plasmid pYLPI7b
• Accession: NZ_CP074035
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: KFU70_RS24510
• Protein ID: WP_016231379.1
• Coordinates: 1803..2183 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KFU70_RS24515
• Protein ID: WP_001190712.1
• Coordinates: 2183..2404 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS24495 (KFU70_24280)	7..459	-	453	WP_016231381.1	hypothetical protein	-
KFU70_RS24500 (KFU70_24285)	548..1591	-	1044	WP_016231380.1	DUF968 domain-containing protein	-
KFU70_RS24505 (KFU70_24290)	1619..1798	-	180	WP_000113019.1	hypothetical protein	-
KFU70_RS24510 (KFU70_24295)	1803..2183	-	381	WP_016231379.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KFU70_RS24515 (KFU70_24300)	2183..2404	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU70_RS24520 (KFU70_24305)	2587..4143	+	1557	WP_016231378.1	type I restriction-modification system subunit M	-
KFU70_RS24525 (KFU70_24310)	4140..4766	+	627	WP_016231377.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90815	90815

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13616.35 Da
• Isoelectric Point: 5.1514

>T201253 WP_016231379.1 NZ_CP074035:c2183-1803 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELVDLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T201253 NZ_CP098247:c422076-421732 [Oxalobacter aliiformigenes]
ATGAAAAAGCAATATTCAATTGATTATTATAATGACGATATTCGACAGGATGTTGACCGTTGGCCGGTTTCTCTCCGTGC
GCGCTACGCCAGACTGACTGAACGCATGAAAATATATGGCGGAAATCTCGGGGAGCCACATACAAAGGCATTCGGGAACG
GACTTTTCGAGTTGCGTGTCAAAGCTGAAGTGGGGATCGGACGCGCTTTTTTCTGCACGAAAGTTGGTCGGAAAATCATT
ATTCTCCACAGCTTTATCAAGAAAACGCAAAAAACGCCGAAGCACGATCTGGATATTGCGTTGTCAAGAATGAAGGAGAA
AAGAGATGAGCAAGACTCACGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT201253 WP_001190712.1 NZ_CP074035:c2404-2183 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT201253 NZ_CP098247:c421751-421467 [Oxalobacter aliiformigenes]
ATGAGCAAGACTCACGATGAAATGGTATCCGAATGGATGAAAGATCCGGCTTTCCGGGAAGAATACGACAAGCTTGAAGC
GGAATTCCGGCTGCTGGATGATATTCTTGCCGCCAGAAATGCGGCTGGCCTGACGCAGGCACAGGTAGCGGAACGCATGG
GAACGACACAATCATCTGTCGCACGGCTTGAGAAGGGGTTAGCATCCGGTAGCTTGCCTTCCATGTCGATGCTGAAACGA
TATGCACAAGTGGTCGGGAAAAAATTGCATGTGCGATTGATATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6