Detailed information of TA system TA201250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 85041..85666
• Replicon: plasmid pYLPI7a
• Accession: NZ_CP074034
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU70_RS24440
• Protein ID: WP_000911313.1
• Coordinates: 85041..85439 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KFU70_RS24445
• Protein ID: WP_000450520.1
• Coordinates: 85439..85666 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS24425 (81366)	81366..81863	+	498	WP_000605859.1	entry exclusion protein	-
KFU70_RS24430 (81916)	81916..82626	+	711	WP_230133085.1	conjugal transfer complement resistance protein TraT	-
KFU70_RS24435 (82879)	82879..85032	+	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KFU70_RS24440 (85041)	85041..85439	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU70_RS24445 (85439)	85439..85666	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(A) / qnrS1 / tet(A)	-	1..96684	96684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T201250 WP_000911313.1 NZ_CP074034:c85439-85041 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T201250 NZ_CP098247:318010-318285 [Oxalobacter aliiformigenes]
ATGGAACTGAAATGGACAAGCAAGGCCGCTTCCGATTTGTCGCGTCTTTATGAATTTCTGGCACCCGCAAACAAAAAAGC
CGCTGCGCGTGCTATACAAGCTTTGATCAAGGCTCCAAACATTCTCTTGACCAACCCGCGAATCAGTGAGCAGCTATTCG
AATTTGAATCACAAGAGGTGAGGCGTATCCTGATAGGAAATTATGAGATACGTTATGAAATAAAGAGTTCGACTATTTTT
GTGCTTCGTGTTTGGCATACACGGGAAAATCGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT201250 WP_000450520.1 NZ_CP074034:c85666-85439 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT201250 NZ_CP098247:317732-318010 [Oxalobacter aliiformigenes]
ATGAAACAGACTCAATCAAAAGTTTTTACGGCGCATGTACCTCTGCCTCTGGCCGATAAAGTCGATCAGCTGGCGGCACG
TATCGAACGTTCGAGAGGCTGGGTGATCAAGCAGGCTTTATCTGCCTGGATAGAGCAGGAAGAAGAACGGTATCAAATGA
CACTTGAAGCGCTTGCCGATGTGGATGCGGGTCGGGTTGTCGATCATGAGGCTATGCTTGCATGGGCGGAAAGTCTGGGC
ACTGATGATCCATTACCTCTGCCGGAGCCTGGGAAATAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2