Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34870..35395 Replicon plasmid pYLPI7a
Accession NZ_CP074034
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KFU70_RS24110 Protein ID WP_001159871.1
Coordinates 35090..35395 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KFU70_RS24105 Protein ID WP_000813630.1
Coordinates 34870..35088 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS24095 (30088) 30088..30273 - 186 Protein_37 hypothetical protein -
KFU70_RS24100 (30499) 30499..34314 + 3816 WP_223364258.1 hypothetical protein -
KFU70_RS24105 (34870) 34870..35088 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KFU70_RS24110 (35090) 35090..35395 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KFU70_RS24115 (35396) 35396..36202 + 807 WP_000016970.1 site-specific integrase -
KFU70_RS24120 (36924) 36924..37679 + 756 WP_000852146.1 replication initiation protein RepE -
KFU70_RS24125 (38267) 38267..39433 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(A) / qnrS1 / tet(A) - 1..96684 96684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T201246 WP_001159871.1 NZ_CP074034:35090-35395 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T201246 NZ_CP098245:1908515-1908793 [Oxalobacter formigenes]
ATGATTAAATCGTTCACATGTCCGGATACACAAGACCTGTTTGCCGGTAAAACCGTTCCCCGGTTTGTCAACATCCGGTC
AGTAGCGGAACGCAAACTCCAGATGATTCACAGGGCTACGGGCATAAACGATTTACGAATTCCACCCAATAACCGCCTTG
AAGCGTTAAAAGGCGATAGAAAAGGTCAATACAGCATAAGAATTAATGACCAGTGGCGCATTTGTTTCACGTTCGATAAT
GGACAGGCTTTTGATGTTTCGATTGTCGATTACCACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT201246 WP_000813630.1 NZ_CP074034:34870-35088 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT201246 NZ_CP098245:1908805-1909104 [Oxalobacter formigenes]
ATGGATAACATTATTAATGGCATGAGGCCAATACATCCGGGGGAAATTCTCAGAGAAGAATTTCTGTCCCCGATGAACTT
GTCTTCCAATGCACTGGCCATTGAACTGCACGTCCCCGCGCCACGGATAAATGAAATTGTCCGTGAACGCAGAAGCATCA
CGGCAGATACCGCTTTACGTCTTGCCCGTTATTTTGGAACAAGTGCTGAATTCTGGATGGGCTTGCAAGATGATTACGAT
CTGAAAACCTGCCAGAGAAAACTCGATGAAACGCTTGGCCGGATTATTCCGCGCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References