Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4490014..4490639 Replicon chromosome
Accession NZ_CP074033
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KFU70_RS22025 Protein ID WP_000911329.1
Coordinates 4490241..4490639 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KFU70_RS22020 Protein ID WP_000450524.1
Coordinates 4490014..4490241 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS21995 (4485816) 4485816..4486286 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KFU70_RS22000 (4486286) 4486286..4486858 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KFU70_RS22005 (4487004) 4487004..4487882 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
KFU70_RS22010 (4487899) 4487899..4488933 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KFU70_RS22015 (4489146) 4489146..4489859 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KFU70_RS22020 (4490014) 4490014..4490241 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KFU70_RS22025 (4490241) 4490241..4490639 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU70_RS22030 (4490786) 4490786..4491649 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KFU70_RS22035 (4491664) 4491664..4493679 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
KFU70_RS22040 (4493753) 4493753..4494451 + 699 WP_000679823.1 esterase -
KFU70_RS22045 (4494561) 4494561..4494761 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T201245 WP_000911329.1 NZ_CP074033:4490241-4490639 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T201245 NZ_CP098244:c638672-638394 [Oxalobacter formigenes]
ATGATTAAATCGTTCACATGTCCGGATACACAAGACCTGTTTGCCGGTAAAACCGTTCCCCGGTTTGTCAACATCCGGTC
AGTAGCGGAACGCAAACTCCAGATGATTCACAGGGCTACGTGCATAAACGATTTACGAATTCCACCCAATAACCGCCTTG
AAGCGTTAAAAGGCGATAGAAAAGGTCAATACAGCATAAGAATTAATGACCAGTGGCGCATTTGTTTCACGTTCGATAAT
GGACAGGCTTTTGATGTTTCGATTGTCGATTACCACTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT201245 WP_000450524.1 NZ_CP074033:4490014-4490241 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT201245 NZ_CP098244:c638382-638083 [Oxalobacter formigenes]
ATGGATAACATTATTAATGGTATGAGGCCAATACATCCGGGGGAAATTCTCAGAGAAGAATTTCTGTCCCCGATGAACTT
GTCTTCCAATGCACTGGCCATTGAACTGCACGTTCCCGCGCCACGGATAAATGAAATTGTCCGTGAACGCAGAAGCATCA
CAGCAGATACCGCTTTACGTCTTGCCCGTTATTTTGGAACAAGTGCTGAATTCTGGATGGGCTTGCAAGATGATTACGAT
CTGAAAACCTGCCAGAGAAAACTCGATGAAACGCTTGGCCGGATTATTCCGCGCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References