Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4040822..4041476 Replicon chromosome
Accession NZ_CP074033
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFU70_RS19875 Protein ID WP_000244772.1
Coordinates 4041069..4041476 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU70_RS19870 Protein ID WP_000354046.1
Coordinates 4040822..4041088 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS19845 (4036110) 4036110..4036853 + 744 WP_000951948.1 SDR family oxidoreductase -
KFU70_RS19850 (4036910) 4036910..4038343 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KFU70_RS19855 (4038388) 4038388..4038699 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFU70_RS19860 (4038863) 4038863..4039522 + 660 WP_257201011.1 hemolysin III family protein -
KFU70_RS19865 (4039599) 4039599..4040579 - 981 WP_000886075.1 tRNA-modifying protein YgfZ -
KFU70_RS19870 (4040822) 4040822..4041088 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU70_RS19875 (4041069) 4041069..4041476 + 408 WP_000244772.1 protein YgfX Toxin
KFU70_RS19880 (4041516) 4041516..4042037 - 522 WP_001055874.1 flavodoxin FldB -
KFU70_RS19885 (4042149) 4042149..4043045 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU70_RS19890 (4043070) 4043070..4043780 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU70_RS19895 (4043786) 4043786..4045519 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T201243 WP_000244772.1 NZ_CP074033:4041069-4041476 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201243 NZ_CP098244:c304924-304652 [Oxalobacter formigenes]
ATGCTGGCATTGAAAACTACTGGCCAATTTCGCAAAGATCTGAAGCTGGCAAAAAAACGTGGATACAAGCTTGAGCTTCT
TGAAGAGGTACTGGAGATACTTCAAAATGAAGAAGCTCTTCCGGCAAAATTTAAGGACCACCCTTTAATCGGCCAATATG
CTGGCTTTCGTGAATGCCACATTCAGCCAGATTGGCTACTGATTTACATCGTCGATAACAATCAGCTTGTTCTGACGACT
GTACGCACAGGTACACATTCTGATTTGTTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201243 WP_000354046.1 NZ_CP074033:4040822-4041088 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201243 NZ_CP098244:c305205-304924 [Oxalobacter formigenes]
ATGGCTAAAGACGCAAACATCAATATTCGTATTGAAAAAGAAGTGAAATCGAATGCTGAAGCATTATTTGCCAGCTTTGG
CATTACTGTTTCGGATGCGATCAAGATATTCCTTCACCAGTCTTTGATGACCGGTGGACTACCTTTTGAAGTACGGCAAC
CTCGTTATAATCGTGAGACTGAATTGGCAATGGATGAAGCAAGACAGATCATTGCAGGAAAGAAGAAATCAAAAAACTAT
CAATCAGCGTCAGACATGATTAACGATCTGGACAGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References