Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3451135..3451721 Replicon chromosome
Accession NZ_CP074033
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KFU70_RS16850 Protein ID WP_042014162.1
Coordinates 3451135..3451365 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KFU70_RS16855 Protein ID WP_000593555.1
Coordinates 3451362..3451721 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS16840 (3447158) 3447158..3449893 + 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -
KFU70_RS16845 (3449893) 3449893..3451017 + 1125 WP_001216257.1 ABC transporter permease -
KFU70_RS16850 (3451135) 3451135..3451365 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU70_RS16855 (3451362) 3451362..3451721 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU70_RS16860 (3451841) 3451841..3452242 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KFU70_RS16865 (3452248) 3452248..3453054 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KFU70_RS16870 (3453051) 3453051..3453815 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KFU70_RS16875 (3453815) 3453815..3454648 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KFU70_RS16880 (3454645) 3454645..3455589 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T201240 WP_042014162.1 NZ_CP074033:3451135-3451365 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T201240 NZ_CP098234:c4840319-4840008 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT201240 WP_000593555.1 NZ_CP074033:3451362-3451721 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT201240 NZ_CP098234:c4840007-4839717 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References