Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2621258..2621853 Replicon chromosome
Accession NZ_CP074033
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KFU70_RS12990 Protein ID WP_000239579.1
Coordinates 2621258..2621608 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A4D0VVQ9
Locus tag KFU70_RS12995 Protein ID WP_001223207.1
Coordinates 2621602..2621853 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS12970 (2616534) 2616534..2617556 - 1023 WP_001296689.1 ABC transporter permease -
KFU70_RS12975 (2617570) 2617570..2619072 - 1503 WP_000205795.1 sugar ABC transporter ATP-binding protein -
KFU70_RS12980 (2619382) 2619382..2620338 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KFU70_RS12985 (2620648) 2620648..2621178 + 531 WP_000055072.1 inorganic diphosphatase -
KFU70_RS12990 (2621258) 2621258..2621608 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KFU70_RS12995 (2621602) 2621602..2621853 - 252 WP_001223207.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KFU70_RS13000 (2622066) 2622066..2622407 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KFU70_RS13005 (2622410) 2622410..2626189 - 3780 WP_047653582.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T201237 WP_000239579.1 NZ_CP074033:c2621608-2621258 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T201237 NZ_CP098234:c4353868-4353518 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9285.61 Da        Isoelectric Point: 4.4201

>AT201237 WP_001223207.1 NZ_CP074033:c2621853-2621602 [Escherichia coli]
MRITIKRWGNSAGMVIPNILMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT201237 NZ_CP098234:c4354113-4353862 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A4D0VVQ9

References