Detailed information of TA system TA201236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2507167..2507981
• Replicon: chromosome
• Accession: NZ_CP074033
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: KFU70_RS12425
• Protein ID: WP_001054376.1
• Coordinates: 2507167..2507424 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: KFU70_RS12430
• Protein ID: WP_001309181.1
• Coordinates: 2507436..2507981 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS12400 (2502455)	2502455..2503561	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
KFU70_RS12405 (2503626)	2503626..2504606	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
KFU70_RS12410 (2504716)	2504716..2504921	+	206	Protein_2428	HNH endonuclease	-
KFU70_RS12415 (2505189)	2505189..2506429	-	1241	Protein_2429	helicase YjhR	-
KFU70_RS12420 (2506545)	2506545..2506676	+	132	WP_001309182.1	hypothetical protein	-
KFU70_RS12425 (2507167)	2507167..2507424	+	258	WP_001054376.1	YjhX family toxin	Toxin
KFU70_RS12430 (2507436)	2507436..2507981	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
KFU70_RS12435 (2508037)	2508037..2508783	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
KFU70_RS12440 (2508952)	2508952..2509170	+	219	Protein_2434	hypothetical protein	-
KFU70_RS12445 (2509208)	2509208..2509324	+	117	Protein_2435	VOC family protein	-
KFU70_RS12450 (2509569)	2509569..2510690	+	1122	WP_000010829.1	M42 family metallopeptidase	-
KFU70_RS12455 (2510687)	2510687..2510965	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
KFU70_RS12460 (2510977)	2510977..2512290	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	2496953..2523671	26718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T201236 WP_001054376.1 NZ_CP074033:2507167-2507424 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T201236 NZ_CP098234:c4251052-4250678 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT201236 WP_001309181.1 NZ_CP074033:2507436-2507981 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT201236 NZ_CP098234:c4251510-4251142 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCTGACGACAATCACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGACATCAGAGGCCGGT
TCAGCGATGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8