Detailed information of TA system TA201234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1888461..1889298
• Replicon: chromosome
• Accession: NZ_CP074033
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KFU70_RS09520
• Protein ID: WP_000227784.1
• Coordinates: 1888756..1889298 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KFU70_RS09515
• Protein ID: WP_001297137.1
• Coordinates: 1888461..1888772 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS09490 (1883481)	1883481..1884428	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KFU70_RS09495 (1884450)	1884450..1886441	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KFU70_RS09500 (1886431)	1886431..1887045	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KFU70_RS09505 (1887045)	1887045..1887374	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KFU70_RS09510 (1887386)	1887386..1888276	+	891	WP_000971336.1	heme o synthase	-
KFU70_RS09515 (1888461)	1888461..1888772	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KFU70_RS09520 (1888756)	1888756..1889298	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KFU70_RS09525 (1889354)	1889354..1890289	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
KFU70_RS09530 (1890697)	1890697..1892061	+	1365	WP_001000975.1	MFS transporter	-
KFU70_RS09535 (1892189)	1892189..1892680	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KFU70_RS09540 (1892848)	1892848..1893759	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T201234 WP_000227784.1 NZ_CP074033:1888756-1889298 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T201234 NZ_CP098234:c3757360-3756983 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGTCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT201234 WP_001297137.1 NZ_CP074033:1888461-1888772 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT201234 NZ_CP098234:c3757818-3757450 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3