Detailed information of TA system TA201211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4366709..4367304
• Replicon: chromosome
• Accession: NZ_CP074031
• Organism: Escherichia coli strain PK12

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: KFU67_RS21310
• Protein ID: WP_000239581.1
• Coordinates: 4366709..4367059 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: KFU67_RS21315
• Protein ID: WP_001223213.1
• Coordinates: 4367053..4367304 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU67_RS21290 (4362163)	4362163..4363185	-	1023	WP_001361374.1	ABC transporter permease	-
KFU67_RS21295 (4363199)	4363199..4364701	-	1503	WP_001095661.1	ATP-binding cassette domain-containing protein	-
KFU67_RS21300 (4364834)	4364834..4365790	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
KFU67_RS21305 (4366100)	4366100..4366630	+	531	WP_000055072.1	inorganic diphosphatase	-
KFU67_RS21310 (4366709)	4366709..4367059	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
KFU67_RS21315 (4367053)	4367053..4367304	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
KFU67_RS21320 (4367516)	4367516..4367857	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
KFU67_RS21325 (4367860)	4367860..4371639	-	3780	WP_000060873.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T201211 WP_000239581.1 NZ_CP074031:c4367059-4366709 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T201211 NZ_CP098231:4174876-4175133 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT201211 WP_001223213.1 NZ_CP074031:c4367304-4367053 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT201211 NZ_CP098231:4175145-4175690 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAGCCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7