Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4355365..4355887 Replicon chromosome
Accession NZ_CP074031
Organism Escherichia coli strain PK12

Toxin (Protein)


Gene name relE Uniprot ID V0SDB6
Locus tag KFU67_RS21250 Protein ID WP_001105432.1
Coordinates 4355365..4355655 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag KFU67_RS21255 Protein ID WP_000212715.1
Coordinates 4355645..4355887 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU67_RS21235 (4350528) 4350528..4352183 + 1656 WP_001361372.1 alpha,alpha-phosphotrehalase -
KFU67_RS21240 (4352577) 4352577..4354715 + 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
KFU67_RS21245 (4354900) 4354900..4355364 + 465 WP_001009180.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
KFU67_RS21250 (4355365) 4355365..4355655 - 291 WP_001105432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFU67_RS21255 (4355645) 4355645..4355887 - 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KFU67_RS21260 (4356079) 4356079..4356465 - 387 WP_001232253.1 cytochrome b562 -
KFU67_RS21265 (4356521) 4356521..4357873 - 1353 WP_001162172.1 metalloprotease PmbA -
KFU67_RS21270 (4357967) 4357967..4358518 + 552 WP_000166270.1 ribosome biogenesis factor YjgA -
KFU67_RS21275 (4358601) 4358601..4359974 - 1374 WP_001219814.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11311.21 Da        Isoelectric Point: 10.0238

>T201210 WP_001105432.1 NZ_CP074031:c4355655-4355365 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYSVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T201210 NZ_CP098231:4128551-4128892 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT201210 WP_000212715.1 NZ_CP074031:c4355887-4355645 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT201210 NZ_CP098231:c4128556-4128480 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SDB6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References