Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4260497..4261073 Replicon chromosome
Accession NZ_CP074031
Organism Escherichia coli strain PK12

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag KFU67_RS20830 Protein ID WP_001131965.1
Coordinates 4260786..4261073 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag KFU67_RS20825 Protein ID WP_000063158.1
Coordinates 4260497..4260799 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU67_RS20805 (4255605) 4255605..4256825 + 1221 WP_000873756.1 amino acid deaminase -
KFU67_RS20810 (4257093) 4257093..4259243 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
KFU67_RS20815 (4259293) 4259293..4259496 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU67_RS20820 (4259507) 4259507..4260463 + 957 WP_001361361.1 GTPase -
KFU67_RS20825 (4260497) 4260497..4260799 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
KFU67_RS20830 (4260786) 4260786..4261073 - 288 WP_001131965.1 BrnT family toxin Toxin
KFU67_RS20835 (4261270) 4261270..4262436 + 1167 WP_000800823.1 restriction endonuclease -
KFU67_RS20840 (4262481) 4262481..4262654 + 174 Protein_4083 type I restriction-modification system subunit M N-terminal domain-containing protein -
KFU67_RS20845 (4262648) 4262648..4263316 + 669 Protein_4084 DUF3387 domain-containing protein -
KFU67_RS20850 (4263531) 4263531..4263869 + 339 WP_000132633.1 endoribonuclease SymE -
KFU67_RS20855 (4263957) 4263957..4264004 - 48 WP_229017048.1 hypothetical protein -
KFU67_RS20860 (4264252) 4264252..4265664 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T201209 WP_001131965.1 NZ_CP074031:c4261073-4260786 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T201209 NZ_CP098231:c4006970-4006656 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT201209 WP_000063158.1 NZ_CP074031:c4260799-4260497 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201209 NZ_CP098231:c4007206-4006973 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References