201208

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3722948..3723785 Replicon chromosome
Accession NZ_CP074031
Organism Escherichia coli strain PK12

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag KFU67_RS18125 Protein ID WP_000227782.1
Coordinates 3723243..3723785 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag KFU67_RS18120 Protein ID WP_001353405.1
Coordinates 3722948..3723259 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU67_RS18095 (3717968) 3717968..3718915 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
KFU67_RS18100 (3718937) 3718937..3720928 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KFU67_RS18105 (3720918) 3720918..3721532 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KFU67_RS18110 (3721532) 3721532..3721861 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KFU67_RS18115 (3721873) 3721873..3722763 + 891 WP_000971327.1 heme o synthase -
KFU67_RS18120 (3722948) 3722948..3723259 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
KFU67_RS18125 (3723243) 3723243..3723785 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
KFU67_RS18130 (3723841) 3723841..3724685 - 845 Protein_3552 sel1 repeat family protein -
KFU67_RS18135 (3725092) 3725092..3726456 + 1365 WP_001000978.1 MFS transporter -
KFU67_RS18140 (3726584) 3726584..3727075 - 492 WP_001138904.1 nucleotide binding protein YajQ -
KFU67_RS18145 (3727243) 3727243..3728154 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
KFU67_RS18150 (3728117) 3728117..3728707 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T201208 WP_000227782.1 NZ_CP074031:3723243-3723785 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T201208 NZ_CP098231:3799196-3799474 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT201208 WP_001353405.1 NZ_CP074031:3722948-3723259 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT201208 NZ_CP098231:3798933-3799193 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References