Detailed information of TA system TA201205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3262349..3263054
• Replicon: chromosome
• Accession: NZ_CP074031
• Organism: Escherichia coli strain PK12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: KFU67_RS15800
• Protein ID: WP_000539524.1
• Coordinates: 3262349..3262735 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KFU67_RS15805
• Protein ID: WP_001280945.1
• Coordinates: 3262725..3263054 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU67_RS15780 (3258353)	3258353..3258979	+	627	WP_001295292.1	glutathione S-transferase GstB	-
KFU67_RS15785 (3258976)	3258976..3260091	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
KFU67_RS15790 (3260202)	3260202..3260585	-	384	WP_000497137.1	biofilm formation regulator BssR	-
KFU67_RS15795 (3260798)	3260798..3262123	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KFU67_RS15800 (3262349)	3262349..3262735	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU67_RS15805 (3262725)	3262725..3263054	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
KFU67_RS15810 (3263124)	3263124..3264441	-	1318	Protein_3104	diguanylate cyclase	-
KFU67_RS15815 (3264449)	3264449..3266797	-	2349	WP_000950331.1	EAL domain-containing protein	-
KFU67_RS15820 (3266974)	3266974..3267885	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T201205 WP_000539524.1 NZ_CP074031:3262349-3262735 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T201205 NZ_CP098231:3548135-3548353 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT201205 WP_001280945.1 NZ_CP074031:3262725-3263054 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT201205 NZ_CP098231:3547735-3548109 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7