Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1139359..1139942 Replicon chromosome
Accession NZ_CP074031
Organism Escherichia coli strain PK12

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag KFU67_RS05455 Protein ID WP_000254738.1
Coordinates 1139607..1139942 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1M2IZI3
Locus tag KFU67_RS05450 Protein ID WP_000581940.1
Coordinates 1139359..1139607 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU67_RS05440 (1135698) 1135698..1136999 + 1302 WP_000046818.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KFU67_RS05445 (1137047) 1137047..1139281 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KFU67_RS05450 (1139359) 1139359..1139607 + 249 WP_000581940.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFU67_RS05455 (1139607) 1139607..1139942 + 336 WP_000254738.1 endoribonuclease MazF Toxin
KFU67_RS05460 (1140014) 1140014..1140805 + 792 WP_001071641.1 nucleoside triphosphate pyrophosphohydrolase -
KFU67_RS05465 (1141033) 1141033..1142670 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KFU67_RS05470 (1142757) 1142757..1144055 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T201194 WP_000254738.1 NZ_CP074031:1139607-1139942 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T201194 NZ_CP098231:c376567-376082 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCATTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9270.67 Da        Isoelectric Point: 4.5972

>AT201194 WP_000581940.1 NZ_CP074031:1139359-1139607 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIGLVDGKLIIEPVRKEPVFTLAELVSDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT201194 NZ_CP098231:c376836-376558 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IZI3

References