Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1016075..1016729 Replicon chromosome
Accession NZ_CP074031
Organism Escherichia coli strain PK12

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KFU67_RS04945 Protein ID WP_000244781.1
Coordinates 1016322..1016729 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU67_RS04940 Protein ID WP_000354046.1
Coordinates 1016075..1016341 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU67_RS04920 (1012163) 1012163..1013596 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
KFU67_RS04925 (1013641) 1013641..1013952 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
KFU67_RS04930 (1014116) 1014116..1014775 + 660 WP_000250269.1 hemolysin III family protein -
KFU67_RS04935 (1014852) 1014852..1015832 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
KFU67_RS04940 (1016075) 1016075..1016341 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU67_RS04945 (1016322) 1016322..1016729 + 408 WP_000244781.1 protein YgfX Toxin
KFU67_RS04950 (1016769) 1016769..1017290 - 522 WP_001521233.1 flavodoxin FldB -
KFU67_RS04955 (1017402) 1017402..1018298 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU67_RS04960 (1018323) 1018323..1019033 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU67_RS04965 (1019039) 1019039..1020772 + 1734 WP_063117505.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T201193 WP_000244781.1 NZ_CP074031:1016322-1016729 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201193 NZ_CP098231:293026-293301 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGACAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201193 WP_000354046.1 NZ_CP074031:1016075-1016341 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201193 NZ_CP098231:293298-293657 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCTCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References