20119

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/-
Location 1008185..1008816 Replicon chromosome
Accession NC_000907
Organism Haemophilus influenzae Rd KW20

Toxin (Protein)


Gene name vapC Uniprot ID Q4QLW0
Locus tag HI_RS05020 Protein ID WP_005651560.1
Coordinates 1008185..1008583 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q4QLV9
Locus tag HI_RS05025 Protein ID WP_005648011.1
Coordinates 1008583..1008816 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI_RS05000 (HI0944) 1003367..1004485 + 1119 WP_005693291.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
HI_RS05005 (HI0945) 1004486..1005508 + 1023 WP_010869093.1 outer membrane-stress sensor serine endopeptidase DegS -
HI_RS05010 (HI0946) 1005582..1006397 - 816 WP_005693293.1 DNA-formamidopyrimidine glycosylase -
HI_RS05015 (HI0946.1) 1006630..1008165 - 1536 WP_005693296.1 L-2,4-diaminobutyrate decarboxylase -
HI_RS05020 (HI0947) 1008185..1008583 - 399 WP_005651560.1 tRNA(fMet)-specific endonuclease VapC Toxin
HI_RS05025 (HI0948) 1008583..1008816 - 234 WP_005648011.1 type II toxin-antitoxin system antitoxin VapB2 Antitoxin
HI_RS05030 (HI0949) 1008963..1010327 - 1365 WP_005693298.1 diaminobutyrate--2-oxoglutarate transaminase -
HI_RS05035 (HI0950) 1010675..1010845 - 171 WP_005613503.1 50S ribosomal protein L33 -
HI_RS05040 (HI0951) 1010857..1011093 - 237 WP_005542826.1 50S ribosomal protein L28 -
HI_RS05045 (HI0952) 1011307..1011972 - 666 WP_010869094.1 DNA repair protein RadC -
HI_RS05050 (HI0953) 1012136..1013338 + 1203 WP_005693302.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(7-56)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15115.46 Da        Isoelectric Point: 8.0514

>T20119 WP_005651560.1 NC_000907:c1008583-1008185 [Haemophilus influenzae Rd KW20]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Download         Length: 399 bp

>T20119 NC_000907:c1008583-1008185 [Haemophilus influenzae Rd KW20]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8880.98 Da        Isoelectric Point: 4.4013

>AT20119 WP_005648011.1 NC_000907:c1008816-1008583 [Haemophilus influenzae Rd KW20]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Download         Length: 234 bp

>AT20119 NC_000907:c1008816-1008583 [Haemophilus influenzae Rd KW20]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q4QLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A806DGS0

References