Detailed information of TA system TA201185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4325628..4326253
• Replicon: chromosome
• Accession: NZ_CP074028
• Organism: Escherichia coli strain PK13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU76_RS20915
• Protein ID: WP_000911330.1
• Coordinates: 4325855..4326253 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU76_RS20910
• Protein ID: WP_000450524.1
• Coordinates: 4325628..4325855 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU76_RS20885 (4321431)	4321431..4321901	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KFU76_RS20890 (4321901)	4321901..4322473	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KFU76_RS20895 (4322619)	4322619..4323497	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU76_RS20900 (4323514)	4323514..4324548	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KFU76_RS20905 (4324761)	4324761..4325474	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU76_RS20910 (4325628)	4325628..4325855	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU76_RS20915 (4325855)	4325855..4326253	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU76_RS20920 (4326400)	4326400..4327263	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
KFU76_RS20925 (4327278)	4327278..4329293	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU76_RS20930 (4329367)	4329367..4330065	+	699	WP_000679823.1	esterase	-
KFU76_RS20935 (4330175)	4330175..4330375	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T201185 WP_000911330.1 NZ_CP074028:4325855-4326253 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T201185 NZ_CP098229:3576730-3576948 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT201185 WP_000450524.1 NZ_CP074028:4325628-4325855 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT201185 NZ_CP098229:3576330-3576704 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33