Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4040190..4040773 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KFU76_RS19505 Protein ID WP_000254745.1
Coordinates 4040438..4040773 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KFU76_RS19500 Protein ID WP_000581937.1
Coordinates 4040190..4040438 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS19490 (4036529) 4036529..4037830 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KFU76_RS19495 (4037878) 4037878..4040112 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KFU76_RS19500 (4040190) 4040190..4040438 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFU76_RS19505 (4040438) 4040438..4040773 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KFU76_RS19510 (4040844) 4040844..4041635 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KFU76_RS19515 (4041863) 4041863..4043500 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KFU76_RS19520 (4043588) 4043588..4044886 + 1299 WP_000036723.1 phosphopyruvate hydratase -
KFU76_RS19525 (4044942) 4044942..4045304 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T201182 WP_000254745.1 NZ_CP074028:4040438-4040773 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T201182 NZ_CP098229:2829534-2829641 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT201182 WP_000581937.1 NZ_CP074028:4040190-4040438 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT201182 NZ_CP098229:c2829487-2829421 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References