Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3903856..3904510 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFU76_RS18890 Protein ID WP_000244772.1
Coordinates 3904103..3904510 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU76_RS18885 Protein ID WP_000354046.1
Coordinates 3903856..3904122 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS18860 (3899144) 3899144..3899887 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU76_RS18865 (3899944) 3899944..3901377 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KFU76_RS18870 (3901422) 3901422..3901733 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
KFU76_RS18875 (3901897) 3901897..3902556 + 660 WP_000250274.1 hemolysin III family protein -
KFU76_RS18880 (3902633) 3902633..3903613 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU76_RS18885 (3903856) 3903856..3904122 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU76_RS18890 (3904103) 3904103..3904510 + 408 WP_000244772.1 protein YgfX Toxin
KFU76_RS18895 (3904550) 3904550..3905071 - 522 WP_001055874.1 flavodoxin FldB -
KFU76_RS18900 (3905183) 3905183..3906079 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU76_RS18905 (3906104) 3906104..3906814 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU76_RS18910 (3906820) 3906820..3908553 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T201181 WP_000244772.1 NZ_CP074028:3904103-3904510 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201181 NZ_CP098229:c2511523-2511347 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201181 WP_000354046.1 NZ_CP074028:3903856-3904122 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201181 NZ_CP098229:c2511301-2510885 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References