Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3307221..3307807 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KFU76_RS15870 Protein ID WP_042014162.1
Coordinates 3307221..3307451 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KFU76_RS15875 Protein ID WP_000593555.1
Coordinates 3307448..3307807 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS15860 (3303258) 3303258..3305993 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
KFU76_RS15865 (3305993) 3305993..3307117 + 1125 WP_001314210.1 ABC transporter permease -
KFU76_RS15870 (3307221) 3307221..3307451 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU76_RS15875 (3307448) 3307448..3307807 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU76_RS15880 (3307927) 3307927..3308328 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KFU76_RS15885 (3308334) 3308334..3309140 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KFU76_RS15890 (3309137) 3309137..3309901 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KFU76_RS15895 (3309901) 3309901..3310734 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KFU76_RS15900 (3310731) 3310731..3311675 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T201178 WP_042014162.1 NZ_CP074028:3307221-3307451 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T201178 NZ_CP098229:2066723-2066826 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT201178 WP_000593555.1 NZ_CP074028:3307448-3307807 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT201178 NZ_CP098229:c2066864-2066593 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References